Composite

Part:BBa_K2810011:Design

Designed by: Ryan Coates   Group: iGEM18_Cardiff_Wales   (2018-09-26)


mCherry under control of the bacterial promoter and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was synthesised as a gBlock and was not put together from separate level 0 parts. This means unlike our other parts in the collection, it does have the Type IIS restriction enzyme sites and sticky ends making it GoldenGate compatible, but it was not made using GoldenGate cloning.


Source

Sequenced as a gBlock. The promoter is from BBa_J23100, RBS from BBa_B0030, and the coding sequence originally RFP from Discosoma, and the promoter from BBa_B0015.

References